Sequence Workbench

An in-platform DNA workbench: edit, analyse and assemble sequences without leaving the workspace. Everything runs on the page against FASTA, GenBank or a saved project, and edits are checkpointed back to workspace storage.

Tools

• Sequence editor — replace, insert, delete, append; annotate regions and extract selections (IUPAC-aware, 1-based coordinates).
• Restriction analysis — cut sites on circular or linear maps, filter by single/all/absent cutters, pick the enzymes to display.
• Primers & PCR — primer design at a target length and Tm, with nearest-neighbour Tm corrected for Na+ and total DNA concentration.
• Translation & ORFs — all six reading frames, adjustable minimum ORF length, optional inline translation beside the bases.
• Pairwise alignment — global or local alignment of two sequences.
• Cloning editor — restriction-based or manual assembly from left/right ends, with a reverse-orientation option.

Views

Circular and linear sequence maps, base-level sequence, feature/annotation tables, restriction-site list and edit history.

Input / output

Import FASTA, GenBank or a saved project; export sequences, and autosave project checkpoints to a workspace file (or work in manual draft mode).